National Dong Hwa University

Biophysical?Mass Spectrometry?Laboratory

Wen-Ping Peng
http://faculty.ndhu.edu.tw/~PENGW/

Research Field

Physics
Introduction

Wen-Ping Peng received his Ph.D. degree (2004) from the Department of Physics at National Taiwan University. He is now a professor at National Dong Hwa University.

My research interest is on the development of novel mass spectrometric methods for rapid identification of viruses, bacteria, cells, and on development of novel ion soft landing techniques using linear ion trap to mass-select high mass biomolecules and nanoparticles. An ambient ion focusing device is also developed to prepare ions in atmospheric conditions with the temperature control on droplets. We have studied ion transmission of high mass biomolecules and nanoparticles using multiple quadrupoles and linear ion trap mass spectrometer, cellular endocytocysis of nano-/micro-particles, algorithm and theory for rapid analysis of electrospray mass spectra of bioparticles, searching biomarkers of clinical bacteria, identification of microorganisms using mass spectrometry techniques, protein/antibody analysis using nanomaterials, MALDI sample preparation, polymer analysis, and development of plasma ionization techniques.

Research Topics

●Ion trap theory and instrumentation
●Pathogen identification using mass spectrometry techniques
●MALDI sample preparation and multiply charged ion formation mechanism
●Electrospray mass spectral interpretation
●Aerosol, viral and bacterial particle detection and mass analysis
●Nanodiamond materials for biomedical applications
●Megadalton MALDI linear ion trap mass spectrometer
●Ambient ion soft landing using electrospray ionization

Honor
  1. Distinguished scholar award of Taiwan Society for Mass Spectrometry 2025.
  2. Young scholar award of Taiwan Society for Mass Spectrometry 2013.
  3. The paper, “Quantitaive measurement of nano-/microparticle endocytosis by cell mass spectrometry” was selected to be highlighted in the frontispiece at the beginning of the communication section by Angewandte Chemie International Edition (2010).
  4. International Journal of Mass Spectrometry Best Student Paper Award-Instrumentation (2004). 
  5. The paper, "Laser-induced acoustic desorption mass spectrometry of single bioparticles", was recognized as a very important paper (VIP) by Angewandte Chemie International Edition (11-24-2005). 
  6. Institute of Atomic and Molecular Sciences annual award for outstanding publication by a junior researcher (2005). 
  7. The achievements of International Journal of Mass Spectrometry Best Student Paper Award (2004) and mass measurement of single bacteria particles were reported by Liberty Times in Taiwan.
  8. The paper published in the Journal of the American Chemical Society 126 (2004): 11766-11767 was recognized as very important paper of Academia Sinica in the year of 2005. 
  9. The paper, "Optical detection methods for mass spectrometry of macroions", was elected as a top 5% paper of IAMS, Academia Sinica during the year of 2003 to 2005
  • Minh Cong Dang, Avinash A. Patil, Ly Khanh Thi Lai, Szu-Wei Chou, Trang Kieu Thi Hoang, Mhar Ian Cua Estayan, Wen-Ping Peng*, Optimization of the Entropy-Based Wavelet Method for Removing Strong RF and AC Interferences in a Charge Detection Linear Ion Trap Mass Spectrometer, Analytical Chemistry, 97, 5066-5076 (2025)
  • Avinash A. Patil, Thị Kh´anh Ly Lại, Ching-Chieh Lee, Yi-Pang Chiu, Zhe-Xuan Liu, Che-Jen Lin, Wen-Ping Peng*, Enhancing mass analysis of ultra-high molecular weight polystyrene: a comparative study of copper and silver salts with MALDI mass spectrometry, Analytical Methods, 17, 1754-1764 (2025) (Cover feature)
  • Po-Chi Soo, Ching-Chieh Lee, Meng-Fu Shie1, Avinash A. Patil, Mhikee Janella N. Descanzo, Ya-Ching Chin, Xi-An Chen, Yu-Tze Horng, Chih-Bin Lin, Jen-Jyh Lee, Cheng-Kang Chiang, Wen-Ping Peng*, Enhancing Sequence Coverage of Nanodiamond-extracted Early Secretory Proteins from Mycobacterium tuberculosis Complex, Analytical Methods, 16, 3464–3474 (2024) (Cover feature)
  • Shao-Yu Liang, Mhar Ian Cua Estayan, Li-Wei Hsieh, Meng-Cheng Pan, Kai Xiang Li, Huan-Cheng Chang, Wen-Ping Peng*, Real-time Monitoring of the Evaporation and Fission of Electrospray-ionized Polystyrene Beads and Bacterial Pellets at Elevated Temperatures, Analytical Chemistry, 96, 18, 7179–7186 (2024) (Cover feature)
  • ‧Avinash A. Patil, Zhe-Xuan Liu, Yi-Pang Chiu, Thị Khánh Ly Lại, Szu-Wei Chou, Chun-Yen Cheng, Wen-Min Su, Hong-Ting Liao, Justin Benedict A. Agcaoili, Wen-Ping Peng*, Development of a linear ion trap mass spectrometer capable of analyzing megadalton MALDI ions, Talanta, 259, 12455 (2023)
  • ‧Shou-Yu Liang, Shih-Chih Wan, Yen-Peng Ho, Yu-Tze Horng, Po-Chi Soo*, Wen-Ping Peng*, Rapid Quantification of Polyhydroxybutyrate Polymer from Single Bacterial Cells with Mass Spectrometry, Analytical Chemistry, 94, 11734-11738 (2022)
  •  Avinash A. Patil, Mhikee Janella N. Descanzo, Justin Benedict A. Agcaoili, Cheng-Kang Chiang, Chia-Liang Cheng, Huan-Cheng Chang, Wen-Ping Peng*, Carboxylated/Oxidized Diamond Nanoparticles for Quantifying Immunoglobulin G Antibodies Using Mass Spectrometry, ACS Applied Nano Materials, 4, 8922-8936 (2021)
  •  Shao-Yu Liang, Avinash A. Patil, Chou-Hsun Han, Szu-Wei Chou, Wen Chang, Po-Chi Soo, Huan-Cheng Chang, Wen-Ping Peng*, Ionization of Submicrometer-Sized Particles by Laser-Induced Radiofrequency Plasma for Mass Spectrometric Analysis, Analytical Chemistry 90, 13236-13242 (2018)
  •  Avinash A. Patil, Cheng-Kang Chiang, Chen-Hao Wen, Wen-Ping Peng*, Forced dried droplet method for MALDI sample preparation, Analytica Chimica Acta, 1031, 128-133 (2018)
     
Educational Background

Ph.D., Department of Physics, National Taiwan University

Job Description

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTC). It is still a significant global health threat. In my laboratory, we have found the  6 kDa early secretory antigenic target (ESAT-6) and 10 kDa culture filtrate protein (CFP-10) can be used as biomarkers for the detection of MTC. We hope to develop the machine Learning technique for Identification of Tuberculosis Biomarkers using mass spectrometry methods. The mass spectrometric data set will be used to train the program. An automatic diagnosis scheme based on the MTC biomarkers will be developed in this project.  

Preferred Intern Educational Level

BS/MSc with the following backgrounds are welcome:

  1. chemistry
  2. nanoscience
  3. physics

Skill sets or Qualities

  1. mass spectrometry analysis
  2. With nano/bio knowledge

Job Description

We have synthesized Materials Institute Lavoisier (MIL)-101(Cr) metal-organic framework (MOF) nanoparticles for the detection of protein molecules in my laboratory. In this study, we hope to modify the composition of MOF nanoparticles to investigate the capture of large protein molecules with mass > 100 kDa. Two mass spectrometric instruments will be employed for the detection of large proteins, i.e., matrix-sssisted laser desorption/ionization (MALDI) time-of-flight mass spectrometer and linear ion trap mass spectrometer. The chemical/physcial properties of MOFs will be examined and tested in this project.

Preferred Intern Educational Level

BS/MSc with the following backgrounds are welcome:

  1. programming/coding 

        2. chemistry

        3. physics

Skill sets or Qualities

  1. algorithm development

        2. mass spectrometry data analysis