Microbial Ecology, Genomics, and Applications Lab
Research Field
Dr. Yin-Tse Huang (黃尹則) is an Assistant Professor in the Department of Biotechnology and Bioindustry Sciences at National Cheng Kung University (NCKU), Taiwan. He obtained his Ph.D. from the University of Florida, USA, specializing in the ecology and evolution of bark beetle–fungus symbioses, and completed postdoctoral training at Kyoto University, Japan, as a Human Frontier Science Program (HFSP) fellow studying insect–fungus mutualistic relationships.
His research integrates mycology, microbial genomics, ecology, and bioinformatics to address fundamental questions about fungal biology and its applications to human health and agriculture. Current focuses include medical mycology of opportunistic pathogens (Scedosporium and Lomentospora), entomopathogenic fungi for biocontrol, and urban soil microbiome ecology using multi-omics approaches.
With research experience across three countries and active collaborations with institutions in Thailand, Australia, the Czech Republic, and the Philippines, Dr. Huang fosters a highly international and interdisciplinary research environment. He has supervised numerous undergraduate and graduate research projects and is committed to mentoring the next generation of scientists in microbial ecology and genomics.
The MEGA Lab (Microbial Ecology, Genomics, and Applications) at the Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, investigates the diversity, ecology, and evolution of fungi and microbial communities and translates these findings into real-world applications in human health and agriculture.
The lab is equipped with modern facilities for molecular biology, microbiology, and bioinformatics, including Oxford Nanopore long-read sequencing, high-throughput amplicon and metagenomic sequencing pipelines, and computational resources for multi-omics data analysis. We maintain extensive fungal culture collections comprising clinical and environmental isolates of medically important species, insect-associated symbiotic fungi, and entomopathogenic strains sourced from diverse ecological niches across Taiwan and internationally.
Our current research spans three major areas: (1) ecology and antifungal resistance of opportunistic fungal pathogens under a One Health framework, (2) development of entomopathogenic fungi for sustainable biocontrol, and (3) microbiome ecology of urban and agricultural soils using multi-omics approaches.
Topic 1: Medical Mycology – Ecology and Antifungal Resistance of Opportunistic Fungal Pathogens
This project investigates the distribution, diversity, and antifungal susceptibility of Scedosporium and Lomentospora species in environmental and clinical settings in Taiwan. Interns will gain experience in environmental sampling, fungal isolation, molecular identification (DNA barcoding, multi-locus sequence typing), antifungal susceptibility testing, and phylogenetic analysis. This topic bridges environmental microbiology with clinical relevance under the One Health framework.
Topic 2: Entomopathogenic Fungi – Genomics and Biocontrol Applications
This project explores the biocontrol potential and genomic underpinnings of entomopathogenic fungi, with a current focus on Purpureocillium takamizusanense against agricultural pests such as the litchi stink bug. Interns will participate in bioassay experiments, fungal stress tolerance studies (UV, temperature, humidity), comparative and functional genomic analyses to understand pathogenicity mechanisms and environmental adaptation, and dual RNA-seq transcriptomic profiling of host–pathogen interactions. This work contributes to the development of genomics-informed, environmentally friendly biopesticides.
Topic 3: Urban and Agricultural Soil Microbiome Ecology
Using metabarcoding (targeting both fungal and bacterial communities) and shotgun metagenomics, this project investigates how land use and human activity shape soil microbial diversity and community structure across Taiwan. Interns will gain hands-on experience in environmental DNA extraction, high-throughput sequencing data processing, bioinformatics pipelines (QIIME2, R, Python), soil chemistry analysis (pH, organic matter, nutrient profiling), and ecological statistics.
• Recruitment and Retention of Exceptional Faculty Award, Ministry of Education, Taiwan (2021, 2022, 2024, 2025)
• Human Frontier Science Program (HFSP) Postdoctoral Fellowship (2019–2021)
• Graduate Research Assistantship Award, University of Florida (2015–2019)
• Travel Awards from Mycological Society of America (2018), University of Florida (2017, 2018)
• Margaret Walton Fellowship, University of Virginia (2016)
• Oral Presentation Award, Taiwan Entomology Society Annual Meeting (2017)
Ph.D., School of Forest, Fisheries, and Geomatics Sciences
University of Florida, Gainesville, Florida, USA (2015–2019)
M.S., Department of Plant Pathology
National Chung Hsing University, Taichung, Taiwan (2009–2011)
B.S., Department of Plant Pathology
National Chung Hsing University, Taichung, Taiwan (2006–2009)
Postdoctoral Training:
Human Frontier Science Program (HFSP) Postdoctoral Fellow, Center for Ecological Research, Kyoto University, Japan (2018–2021). Research focused on ecological and molecular determinants of specificity in bark beetle–fungus mutualistic symbioses.
Job Description
This project investigates post-disaster shifts in soil microbial communities and their implications for agricultural resilience in watersheds affected by landslide dam breach and high-sediment flood events. Massive sediment deposition and landscape disturbance fundamentally alter soil physicochemical properties and disrupt native microbiome structure, with cascading consequences for soil fertility, plant health, and long-term agricultural productivity. Interns will participate in systematic on-site soil and sediment sampling across disturbed and reference sites, DNA extractions optimized for high-sediment and clay-rich matrices, and shotgun metagenomic sequencing using both Illumina short-read and Oxford Nanopore long-read platforms to capture microbial community composition, functional gene repertoires, and resistome dynamics. Bioinformatics analyses will include taxonomic profiling, functional annotation, and comparative analysis between disaster-affected and control sites to identify microbial indicators of soil recovery and agricultural resilience. This work contributes to building an evidence base for microbiome-informed post-disaster land restoration and food security strategies in disaster-prone watersheds.
Preferred Intern Educational Level
Undergraduate/gradute students are all welcome
Skill sets or Qualities
Environmental sampling
Aseptic operations
DNA extration