Our laboratory current topics are:

1. Apply cell allotransplantation technology to transplant five types of human lung cancer cells and three types of ovarian cancer cells into zebrafish embryos. These cells are transfected with Desmocollin 2 (DSC2), BRCA1, BRCA2, BARD1, and RAD51 shRNA vectors to knock down their genes. The study focuses on determining the optimal transplantation location, cell number, and the effects of cancer cell proliferation, migration, and metastasis within the zebrafish, as well as the impact on the zebrafish's development. This establishes a live zebrafish culture analysis system. The development and results of this research method not only provide a reference and collaboration space for related studies but will also be expanded for cancer drug.
2. The changes in the body color of fish are known to be related to the interaction of nerves, various endogenous steroids and proteins, and chromatophores (pigment cells).  The endogenous electric field within an organism is highly correlated with its development, regeneration, and cell physiology, and is even associated with cancer. However, there has been little research on the effects of electric fields on fish body color. Our laboratory has found that the morphology and pigmentation of black and red pigment cells in the fins of tilapia (Oreochromis mossambicus)  and zebrafish (Danio rerio) can be altered by electrical stimulation of varying intensities. However, no reports currently explain the underlying mechanism.  We will investigate the effects of different voltages, electric shock durations, and frequencies on the morphology (pigment aggregation or dispersion), pigmentation (area, spectrum, and melanin content), and the expression of related genes (tyrosinase, sox10, Mitf, and dct) in the pigment cells of tilapia and zebrafish.