Innovative Bioproducts Laboratory
Research Field
Dr. Doan's academic journey began at Nha Trang University, Vietnam, where she earned her Bachelor's degree in Biotechnology in 2012. Building upon her foundation, she pursued further studies at the National Pingtung University of Science and Technology, Taiwan. There, she completed her Master's and Ph.D. degrees in Animal Vaccine Technology in 2020. Currently, she is an assistant professor in the International Degree Program in Animal Vaccine Technology at the International College, National Pingtung University of Science and Technology. Her current research interest lies in animal vaccine and adjuvant development.
Welcome to Dr. Doan's laboratory, where innovative research converges with a commitment to enhancing animal health. Our team is dedicated to developing vaccines for chickens, ducks and pigs. To enhance vaccine efficacy, we are focusing on Toll-like receptor ligands as biological adjuvants, including TLR5 ligands like flagellin, TLR4 ligands such as suilysin, and TLR2 ligands like Lipoprotein. With technological advancements, our scope also includes genetic engineering and protein expression systems utilizing E. coli, baculovirus, and mammalian cells. Join us as we push the boundaries of veterinary science and strive to safeguard animal populations worldwide.
- Animal vaccines (for chickens, ducks and pigs)
- Biological adjuvants (Bacterium-like particles, Toll-like receptor ligands)
- Expression systems (E. coli, Baculovirus, Mammalian)
Principal projects:
- Identification of Immunogenic domains of leukotoxin for better subunit vaccine (114/01/04-114/12/31)
- Generation of gram-positive enhancer matrix (GEM) particles displaying immunostimulant adjuvants (113/01/04-113/12/31)
- Novel application of bioinformatics in biological adjuvant development (112/01/04-112/12/31)
- Cloning and expression of truncated Streptococcus suis suilysin, along with Protein Anchor as an immunostimulant adjuvant for vaccine development (2024/1/1-2024/12/31)
- Identification and phylogenetic analysis of a novel duck parvovirus in Viet Nam (2021/1/1 – 2021/12/31)
- Study on the expression levels of the truncated domain of duck egg drop syndrome virus (DEDSV) Envelope (E) protein in a prokaryotic system (2020/1/1-2020/12/31)
Publications since 2020.
1. T.D. Phuong Nguyen, P. Thuan Cu, Teerajet Laohasatian, Thu-Dung Doan* (2025, Dec). Immunological aspects of mucosal vaccine-based bacterial-like particles (BLPs) – An updated review. Microbial Pathogenesis. 本人為通訊作者.
2. Haroon Afzal, Li-Ting Cheng, Nguyen-Thanh Hoa, Chun-Yen Chu, Thu-Dung Doan* (2025, Nov). In silico prediction and comparative analysis of full-length and truncated forms of duck Tembusu virus envelope proteins expressed in E. coli and baculovirus systems. British Poultry Science. 本人為通訊作者.
3. Thu-Dung Doan, Teerajet Laohasatian, Hsing-Chieh Wu, Chun-Yen Chu (2025, Oct). Protective efficacy of the recombinantly expressed C-terminal domain of Mannheimia haemolytica leukotoxin in mice and goats. Journal of Veterinary Research. 本人為第一作者.
4. Haroon Afzal, Nguyen-Thanh Hoa, Asad Murtaza, Thu-Dung Doan, Yao-Chi Chung, Guan-Ming Ke, Li-Ting Cheng (2025, Jun). Conserved domains of flagellin as adjuvants for an inactivated vaccine against porcine epidemic diarrhea virus. The Veterinary Journal, Volume 313, 106393.
5. Hoa Nguyen Thanh, Haroon Afzal, Thu-Dung Doan, Asad Murtaza, Chia-Hung Yen, Yao-chi Chung (2025, Feb). Utilizing NF-κB Signaling in Porcine Epithelial Cells to Identify a Plant-Based Additive for the Development of a Porcine Epidemic Diarrhea Virus Vaccine. Veterinary Sciences
6. Hsing-Chieh Wu1†, Thu-Dung Doan†, Wan-Chen Chang, Min-Kung Hsu, Hsian-Yu Wang, Jiahorng Liaw, Chia-Jung Chang, Chun-Yen Chu (2025). Prevention of porcine epidemic diarrhea virus with nanotube-adjuvanted oral DNA vaccines. Transboundary and Emerging Diseases. (Accepted). 本人為第一 作者.
7. Hoa Nguyen Thanh, Haroon Afzal...Thu-Dung Doan, Chung Yao-chi (2024, Sep). Enhanced Immune Response with Baculovirus-Expressed BoHV-1 Glycoprotein D in Vaccine Development. The Veterinary Journal.
8. Hafiz Abdul Haseeb, Hassam Rasheed, Muhammad Usman Zahid, Thu-Dung Doan… and Muhammad Aslam Khan (2024, Jun). Polygonum bistorta Linn. as a green source for synthesis of biocompatible selenium nanoparticles with potent antimicrobial and antioxidant properties. BioMetals.
9. Yao-Chi Chung, Li-Ting Cheng, Chun-Yen Chu, Haroon Afzal, Thu-Dung Doan* (2024, Jun). Flagellin enhances the immunogenicity of Pasteurella multocida lipoprotein E subunit vaccine. Avian Diseases. 本人為通訊作者.
10. Van-Nui Nguyen, Trang-Thi Ho, Thu-Dung Doan, Nguyen Quoc Khanh Le (2024, May). Using a hybrid neural network architecture for DNA sequence representation: A study on N4- methylcytosine sites.
11. Li-Ting Cheng, Chun-Yen Chu, Hung Vu-Khac and Thu-Dung Doan* (2023, Jan). Signal sequence contributes to the immunogenicity of Pasteurella multocida lipoprotein E. Poultry Science, 102. 本人為通訊作者.
12. Asad Murtaza, Haroon Afzal, Thu-Dung Doan, Guan-Ming Ke and Li-Ting Cheng (2022, Oct). Flagellin Improves the Immune Response of an Infectious Bursal Disease Virus (IBDV) Subunit Vaccine. Vaccines, 10, 1780.
13. Min-Chia Wu1, Thu-Dung Doan1, Jai-Wei Lee, Yi-Ting Lo, Hsing-Chieh Wu and Chun-Yen Chu (2022, Aug). Recombinant suilysin of Streptococcus suis enhances the protective efficacy of an engineered Pasteurella multocida toxin protein. Research in Veterinary Science, 151, 175–183. 本人為第一作者.
14. Thu-Dung Doan, Hsian-Yu Wang, Guan-Ming Ke and Li-Ting Cheng (2020, Jun). N-terminus of Flagellin Fused to an Antigen Improves Vaccine Efficacy against Pasteurella multocida Infection in Chickens. Vaccines, 8, 283. 本人為第一作者.
A book chapter:
Thu-Dung Doan, Hsing-Chieh Wu, and Chun-Yen Chu. Preparation and Study of Bacterins. Streptococcus suis Methods and Protocols. UK: Springer Protocols. Jun, 2024: 131-141. 本人為第一作者
International conferences in 2024-2025
1. Duc Thai Ha Nguyen, Hsing Chieh Wu, Thu-Dung Doan* (2025, Dec). Africa swine fever control and prevention: an update on vaccine development in Vietnam. 中華民國獸醫學會秋季研討會暨會員大會. 本人為通訊作者.
2. Thu-Dung Doan* (2025, Nov). Human Resource Development in the fields of Animal Science and Veterinary Medicine in the Digital Era at Tropical Agricultural Universities. The 6th National Conference on Animal and Veterinary Sciences (AVS2025). 本人為第一作者、通訊作者.
3. M. T Tran, Haroon Afzal, Thu-Dung Doan* (2025, Aug). Application of bioinformatics in designing protein-based vaccines. Vietnam International Conference on Genome Biology (VICGB 2025). Quy Nhon, Vietnam. 本人為通 訊作者.
4. Thu-Dung Doan* (2025, Jun). Green Nanotechnology for Animal Vaccines: A Sustainable Approach to Disease Prevention. The 11th International Seminar on Sustainable Agriculture, Food and Energy (SAFE 2025). Ha Noi, Vietnam. 本人 為第一作者、通訊作者.
5. Thu-Dung Doan*, Hsing-Chieh Wu (2025, Jun). Green Nanotechnology for Animal Vaccines: A Sustainable Approach to Disease Prevention. The 11th International Seminar on Sustainable Agriculture, Food, and Engergy (SAFE2025). 本人為第一作者、通訊作者.
6. Thu-Dung Doan*, Hsing-Chieh Wu, Chun-Yen Chu (2024, Nov). Cloning and expression of truncated streptococcus suis suilysin for vaccine adjuvant development. The 2024 Celebrating Centennial NPUST International Conference on Sustainability. 本人為第一作者、通訊作者.
7. Thu-Dung Doan*, Hsing-Chieh Wu, Chun-Yen Chu (2024, Oct). Displaying Immunostimulants on Gram-Positive Enhancer Matrix (GEM) Particles as Mucosal Adjuvants. 7th INTERNATIONAL BIOLOGY CONFERENCE 2024, Vietnam. 本人為第一作者、通訊作者.
8. Thu-Dung Doan*, M. T Tran, Hsing-Chieh Wu, Li-Ting Cheng and Chun-Yen Chu (2024, Sep). Novel surface display system for proteins on gram-positive enhancer matrix (GEM) particles as vaccine adjuvant. The 4th Joint Meeting in Veterinary Science in East Asia, Japan. 本人為第一作者、通訊作者.
9. Thu-Dung Doan*, Yao-Chi Chung, Chun-Yen Chu and Li-Ting Cheng (2024, Sep). Flagellin boosts immunogenicity of Pasteurella multocida lipoprotein E subunit vaccine. The 4th Joint Meeting in Veterinary Science in East Asia. 本人 為通訊作者.
Patents:
- No. I897612: 嵌合型抗原及含此的豬用次單位疫苗組成物 (2025/9/11-2044/9/5)
- No. I845857: 生物性油質佐劑及其製造方法和應用 (2024/6/21-2041/8/12
- No. I782542: 一種鞭毛蛋白的重組蛋白及其用途 (2022/11/1-2041/5/24)
- No. I768816: 自走式農作物噴灌裝置 (2022/6/21-2041/4/6
Technology transfers:
- A process for cloning VP2 gene of the novel duck Parvovirus in Vietnam – Institute for Veterinary Research and Development of Central Vietnam (IVRD) – Vietnam – 2022/07/08 – 2023/07/08.
- 豬冠狀病毒口服DNA疫苗 – 瑞寶基因股份有限公司 (編號:TTN-113-032) (2024/12/1-2025/12/1)
Awards:
- NSTC's 2024 Future Tech Awards: Development of new generation vaccines against swine corona virus. Oct 19, 2024.
- Oral Presentation Prize Winner Veterinary Association Congress, at the Online Avian Research Symposium 2022 – The Roslin Institute, The Royal (Dick) School of Veterinary Studies, UK.
- Certificate of Recognition for Valuable Contributions in Quang Nam Province, Vietnam, June 7, 2022.
- Certificate of Outstanding Achievement in 2022, Provided by Vietnam Economic and Culture Office in Taipei.
- Certificate of Outstanding Achievement in 2020, Provided by Vietnam Economic and Cultural Office in Taipei, Taiwan.
- Awarded Taiwan Scholarship for a doctor’s degree by the Ministry of Education of the Republic of China (Sept. 2016- Aug. 2020)
- Sept. 2016 – Jul. 2020 National Pingtung University of Science and Technology, Doctor of International Degree Program in Animal Vaccine Technology
- Sept. 2013 – Jul. 2015 National Pingtung University of Science and Technology, Master of The Graduate Institute of Animal Vaccine Technology
- Sept. 2008 – Jul. 2012 Nha Trang University, Vietnam, Bachelor of Biotechnology
Job Description
You will also have the opportunity to gain hands-on experience in molecular laboratory techniques. Throughout the program, interns will stay up to date with the latest advancements in vaccine development technologies.
Preferred Intern Educational Level
Master’s or Bachelor’s students with a strong record of research achievements and publications in the fields of biotechnology, veterinary science, or animal vaccine development.
Skill sets or Qualities
Ability to conduct molecular laboratory techniques and immunological assessments, including:
Genetic engineering
Protein expression using the E. coli expression system
Protein analysis by SDS-PAGE and Western blotting
Transmission electron microscopy (TEM) assays
Immunization procedures and immune evaluation in animal models
Job Description
You will also have the opportunity to gain hands-on experience in molecular laboratory techniques. Throughout the program, interns will stay up to date with the latest advancements in vaccine development technologies.
Preferred Intern Educational Level
Master’s or Bachelor’s students with a strong record of research achievements and publications in the fields of biotechnology, veterinary science, or animal vaccine development.
Skill sets or Qualities
Ability to conduct molecular laboratory techniques and immunological assessments, including:
Genetic engineering
Protein expression using the E. coli expression system
Protein analysis by SDS-PAGE and Western blotting
Transmission electron microscopy (TEM) assays
Immunization procedures and immune evaluation in animal models